Taq Man Real Time PCR detection of Listeria monocytogenes: A study of enrichment incubation time affecting sensitivity in experimental dry fermented sausages
نویسندگان
چکیده
منابع مشابه
Presence of Listeria monocytogenes in Mediterranean-Style Dry Fermented Sausages
The morphological, physiological and epidemiological features of L. monocytogenes, together with the severity of human listeriosis infections, make L. monocytogenes of particular concern for manufacturers of cold-stored "ready to eat" (RTE) foods. L. monocytogenes has been isolated from a wide variety of RTE foods and is responsible for several outbreaks associated with the consumption of RTE m...
متن کاملRapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR.
A rapid method for the detection of Listeria monocytogenes in foods combining culture enrichment and real-time PCR was compared to the ISO 11290-1 standard method. The culture enrichment component of the rapid method is based on the ISO standard and includes 24h incubation in half-Fraser broth, 4h incubation in Fraser broth followed by DNA extraction and real-time PCR detection of the ssrA gene...
متن کاملQuantitative detection of Listeria monocytogenes in biofilms by real-time PCR.
A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
متن کاملRapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detecti...
متن کاملDetection of Listeria monocytogenes in Fresh Produce Using Molecular Beacon—Real-time PCR Technology
The capability of an assay to detect Listeria monocytogenes from artificially inoculated fresh-cut produce such as cantaloupe and mixed salad was demonstrated. An oligonucleotide probe that becomes fluorescent upon hybridization to the target DNA (Molecular Beacon, MB) was used in a real-time polymerase chain reaction (PCR) assay. As few as 4 to 7 colony-forming units (CFU) of L. monocytogenes ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Tehnologija mesa
سال: 2014
ISSN: 0494-9846
DOI: 10.5937/tehmesa1401060l